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Escherichia coli

Escherichia coli
Photo Information
Copyright: Paul Braynard (pbraynard) Silver Star Critiquer/Gold Note Writer [C: 44 W: 0 N: 96] (891)
Genre: Monera
Medium: Color
Date Taken: 2011-01-28
Categories: Single-celled
Camera: Nikon Coolpix P90
Photo Version: Original Version
Date Submitted: 2011-01-28 23:51
Viewed: 4725
Points: 4
[Note Guidelines] Photographer's Note
I work in a laboratory where a variety of human samples are cultured for pathogenic organisms. We perform what is referred to as a culture and sensitivity. We identify the organism and identify antibiotics that inhibit the growth of that organism. This is a view of a MacConky agar plate with Escherichia coli growing on it. Here the agar plate has been streaked for isolation. Note the few individual colonies to the left. Each colony contains thousands of organisms. Pure individual colonies are required to perform both the identification and sensitivity. After inoculating the plates, they are incubated in a CO2 incubator at 37 degrees C. E. Coli is known as a gram negative organism. When gram stained the organism is pink. MacConkey agar is a special media used to inhibit gram positive organisms such as staph and step, while allowing gram negative organisms to flourish. Staph and Step are both gram positive, and stain blue or purple.

I hope you find this photo of interest. I'm not certain if it complies with TN standards and figure I'll find out pretty soon. I do wish I could mount my camera to one of our microscopes, but I don't have that capability.

Best regards,


petrudamsa, oanaotilia has marked this note useful
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Ciao Paul, my doday neighbour, very inusual and amazing macro with fantastic details, I think this is the first time of this kind of photo on TN, but I think it is appropriate, it's a form of life, wonderful light and excellent sharpness, very well done my friend, have a good week end, ciao Silvio

Yes, it is interesting to see the plates, much better if we could look down your microscope! regards Yiannis

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